This is supported by the Program Project on Genomics and Proteomics, Academia Sinica, and National Science and Technology Program for Agricultural Biotechnology, Taiwan.
This program project focuses on rice functional genomics. Rice is a great material as a crop for functional genomics research. Taiwan joins the international effort to decode rice genome, and works on the genome sequencing project of chromosome 5. A central lab in Institute of Botany works on this project. As a consequence, we have the ability and capacity on high-throughput sequencing and we may share the most recent database from the international effort. The rice functional genomics research in this campus is thus essential. The PIs/coPIs joining this project include researchers from Institute of Botany, Institute of Molecular Biology, Taiwan Agriculture Research Institute and National Seed Stock Center who major in molecular biology, plant physiology, genetics, plant breeding, and so on.
Knock-out mutants (naturally occurring alleles, deletion mutants or insertion mutants) are the central dogma of functional genomics study for all organisms. Most of the component projects in this program project focus on this topic. For establishment of the mutant population, generation of genome-wide gene knockout by T-DNA, flanking sequence analysis, seed collection and phenotype characterization, seed conservation and PCR screening are included. We also establish a database of the insertion mutant population. Along with the on-going projects such as genome sequencing, EST projects, SAGE study, bioinformatics and chromosome localization, this functional genomics team will accelerate the rice research and usage in Taiwan.